The effect of type I IFN treatment on global gene expression in monocytes undergoing maturation. The effect of type I IFN treatment on global gene expression in monocytes undergoing maturation

To analyze the effect of type I IFN on global gene expression in differentiating monocytes, we employed microarray-based gene expression profiling. The MACS-purified mouse BM monocytes were cultured in M-CSF and treated with or without IFN for 60 h. The cells were harvested and isolated RNAs were subjected to microarray analyses. Overall design: MACS-purified monocytes from mouse bone marrow were divided into two groups (each in triplicates) and treated with 20 ng/mL M-CSF, together with or without 100 U of mouse IFN-beta for 60 h. The resulting cultures were harvested and the RNA samples were subjected to microarray analyses.

为探究I型干扰素（type I IFN）对分化中单核细胞的全局基因表达的影响，我们采用了基于微阵列的基因表达谱分析技术。经磁激活细胞分选（MACS）纯化的小鼠骨髓单核细胞，于巨噬细胞集落刺激因子（M-CSF）培养体系中培养，并分别施加干扰素处理与空白对照，处理时长为60小时。收集细胞后，提取的RNA样本将用于微阵列分析。
总体实验设计：将经MACS纯化的小鼠骨髓单核细胞分为两组（每组设置3次生物学重复），两组均添加20 ng/mL的M-CSF，其中一组额外加入100 U小鼠β干扰素（IFN-β），共同培养60小时。收集两组培养物，提取RNA样本后进行微阵列分析。