Glucose supply and glycolysis inhibition shape the clinical fate of preterm newborns infected with Staphylococcus epidermidis [cord blood]

To investigate the glycolysis inhibition effect on the inflammation responses of preterm newborns infected with Staphylococcus epidermidis, we used preterm pigs` cord blood to mimic the inflammation condition of preterm newborns at birth and stimulated with S. epidermidis and glycolysis inhibior. We then performed gene expression profiling analysis using data obtained from RNA-seq of the cord blood, which were stimulated with S. epidermidis and DCA. Comparative gene expression profiling analysis of RNA-seq data for cord blood samples with/without S. epidermidis (5×10^5 CFU/ml) and DCA incubation.

为探究糖酵解抑制对表皮葡萄球菌（Staphylococcus epidermidis）感染早产新生儿炎症应答的影响，本研究以早产仔猪脐带血模拟出生时早产新生儿的炎症状态，分别以表皮葡萄球菌与糖酵解抑制剂对脐带血进行刺激。随后，基于经表皮葡萄球菌与二氯乙酸（DCA）刺激的脐带血RNA测序（RNA-seq）数据开展基因表达谱分析；本研究同时对经/未经5×10^5 菌落形成单位/毫升（CFU/ml）表皮葡萄球菌及DCA孵育的脐带血样本的RNA测序数据进行对比基因表达谱分析。