Genome-wide transcriptome analysis of the ovaries in different sheep

We performed a genome-wide analysis of mRNAs and lncRNAs from Small Tail Han sheep of genotypes FecBBFecBB (Han BB) and FecB+ FecB+ (Han++) and from Dorset sheep (Dorset) to identify potential regulators of fecundity. An integrated analysis revealed significantly correlated patterns of expression. Dramatic changes of mRNAs and lncRNAs suggest their critical roles in sheep fecundity. This study provides a novel view of the regulatory mechanisms involved in sheep fecundity. Overall design: Ovary transcritomic profiles of different sheep were generated by RNA-Seq, in triplicate, using Illumina HiSeq 2000.

本研究针对三种基因型绵羊的信使RNA（mRNA）与长链非编码RNA（lncRNA）开展全基因组分析，受试绵羊分别为FecBBFecBB型小尾寒羊（Han BB）、FecB+FecB+型小尾寒羊（Han++）以及杜泊羊（Dorset），旨在筛选调控绵羊繁殖力的潜在因子。整合分析结果显示，不同转录本间存在显著相关的表达模式；信使RNA与长链非编码RNA的表达量出现剧烈变化，提示二者在绵羊繁殖调控过程中扮演关键角色。本研究为解析绵羊繁殖力相关调控机制提供了全新视角。实验整体设计：采用Illumina HiSeq 2000平台开展RNA测序（RNA-Seq），对不同绵羊的卵巢转录组进行检测，每组设置三次生物学重复。