Phylogenetic species delimitation for the widespread spider wasp Ageniella accepta (Hymenoptera: Pompilidae), with new synonyms: supplementary material

<i>Ageniella</i> is the second-most diverse spider wasp genus in Ageniellini (Pepsinae). The <i>Ageniella</i> (<i>Ageniella</i>) <i>accepta</i> species-group is found from Canada to Panama and is composed of three Nearctic species: <i>A. accepta </i>(Cresson), <i>A. blaisdelli</i> (Fox), and <i>A. conflicta</i> Banks. Within this group, species-level identification is difficult, because diagnostic characters are questionable, and subjective for both males and females. Furthermore, sexes of each species are not reliably associated. Herein, we investigate sex associations and the validity of described species within the <i>A. accepta </i>species-group based on three molecular markers (cytochrome oxidase I, wingless, long-wavelength rhodopsin) by using maximum likelihood and Bayesian phylogenetic analyses, and species-delimitation approaches. Additionally, we mapped 12 morphological traits onto the molecular phylogeny to discuss evolution of diagnostic characters. We concluded that the three species of the <i>A. accepta</i> species-group are actually a single, wide-ranging species with strong geographical signal. Moreover, our results suggest introgression at the mitochondrial level.

阿格蛛蜂属（Ageniella）是蛛蜂亚科（Pepsinae）阿格蛛蜂族（Ageniellini）中物种多样性排名第二的属。接受阿格蛛蜂（Ageniella accepta）物种群分布范围涵盖加拿大至巴拿马一带，由3个新北界物种组成：接受阿格蛛蜂（A. accepta，Cresson）、布莱斯代尔阿格蛛蜂（A. blaisdelli，Fox）以及冲突阿格蛛蜂（A. conflicta，Banks）。该类群的物种级鉴定难度极高，因其鉴别特征存疑，且雌雄个体的形态判定均带有主观性。此外，各物种的雌雄个体亦无法实现可靠关联。本研究基于细胞色素氧化酶I、无翅基因（wingless）以及长波长视紫红质基因这3个分子标记，采用最大似然法与贝叶斯系统发育分析，并结合物种界定方法，对接受阿格蛛蜂物种群的雌雄个体关联问题以及已描述物种的有效性展开探究。同时，本研究将12项形态性状映射至分子系统发育树，以探讨鉴别特征的演化规律。研究结论显示，接受阿格蛛蜂物种群的3个物种实际上为一个广布物种，且具备显著的地理分布信号。此外，研究结果还表明该类群存在线粒体水平的基因渐渗现象。