Heteromita sp. PRA-74 strain:ATCC-PRA-74 Raw sequence reads

Eukaryotes existed exclusively as microorganisms for a minimum of 500,000 to one billion years prior to the evolution of plants, animals and fungi. Understanding the evolutionary history of microbial eukaryotes, especially the protists, is key to understanding the origins of eukaryotes, the emergence of parasitism, and the evolution of this remarkably diverse assemblage into plants, animals, fungi and other recently derived groups. Protists represent an eclectic group of organisms marked by numerous innovations in body plans, ecology, biochemistry, and molecular processes. The transition from prokaryotic life-forms to eukaryotic cellular architecture, and the origins of multi-cellular eukaryotes are rooted in the world of protists. project describes the collection of new molecular data and the construction of a large-scale, multi-gene data set for exploring evolutionary relationships for diverse eukaryotes. Our experimental strategy seeks to capture the diversity of eukaryotes by exploring relationships within major clades, and by placing taxa of uncertain position on genealogies. We constructed cDNA libraries for over 20 key protists and sequenced 10,000-20,000 cDNA clones from each library. PI Mitchell L. Sogin Marine Biological Laboratory

在植物、动物与真菌演化出现之前，真核生物（Eukaryotes）曾完全以微生物形式存续，时长至少为50万年至10亿年。解析微生物真核生物，尤其是原生生物（protists）的演化历史，是理解真核生物起源、寄生现象的出现，以及这一高度多样的类群如何演化形成植物、动物、真菌与其他新近衍生类群的核心关键。原生生物是一类兼具多样特征的生物类群，其在躯体构型、生态学、生物化学及分子过程领域均存在诸多创新性演化。从原核生命形式到真核细胞结构的演化转变，以及多细胞真核生物的起源，均植根于原生生物的演化谱系之中。本项目旨在采集新型分子数据，并构建大规模多基因数据集，以解析多样真核生物的演化亲缘关系。本实验策略通过探究主要演化支（clades）内部的亲缘关系，并将分类位置未定的类群锚定至演化谱系树中，以全面覆盖真核生物的多样性。我们为20余种关键原生生物构建了cDNA文库，并从每个文库中测序得到10000至20000个cDNA克隆。项目负责人：Mitchell L. Sogin，海洋生物实验室（Marine Biological Laboratory）