Raw data obtained from multi-electrode electrophysiological recordings of hippocampal slices

Preclinical and clinical studies show that mild to moderate hypothermia is neuroprotective in sudden cardiac arrest, ischemic stroke, perinatal hypoxia/ischemia, traumatic brain injury and seizures. Induction of hypothermia largely involves physical cooling therapies, which induce several clinical complications, while some molecules have shown to be efficient in pharmacologically induced hypothermia (PIH). Neurotensin (NT), a 13 amino-acid neuropeptide that regulates body temperature, interacts with various receptors to mediate its peripheral and central effects. NT induces PIH when administered intracerebrally. However, these effects are not observed if NT is administered peripherally, due to its rapid degradation and poor passage of the blood brain barrier (BBB). We conjugated NT to peptides that bind the low-density lipoprotein receptor (LDLR) to generate “vectorized” forms of NT with enhanced BBB permeability. We evaluated their effects in epileptic conditions following peripheral a..., Hippocampal slices (350 μm thick) were prepared from Sprague Dawley rats (n=3; 3-4 weeks old; Janvier Laboratories) and cut using a vibratome (Leica VT1200S) in an ice-cold oxygenated, modified ACSF, continuously aerated with 95% O2 and 5% CO2 and containing Glucose 11 mM, NaHCO3 25 mM, NaCl 126 mM, KCl 3.5 mM, NaH2PO4 1.2 mM, MgCl2 1.3 mM and CaCl2 2 mM. Slices were then incubated at RT for at least 1 H in ACSF. Recordings were performed on hippocampal slices using multielectrode arrays (MEA). Slices were continuously perfused with the oxygenated ACSF at the rate of 3 mL/min with a peristaltic pump (MEA chamber volume: ~1 mL). KA (300 nM) and VH-N412 (0.1, 1 and 10 μm) were added to the perfusion solution to assess the effects of VH-N412 compound on KA-induced increase of neuronal firing. Complete solution exchange in the MEA chamber was achieved 20 s after the switch of solutions. The perfusion liquid was continuously pre-heated at 37°C just before reaching the MEA chamber with a heat..., , # Raw data obtained from multi-electrode electrophysiological recordings of hippocampal slices

[https://doi.org/10.5061/dryad.vdncjsz5k](https://doi.org/10.5061/dryad.vdncjsz5k)

## Description of the data and file structure

**Raw data obtained from multi-electrode recordings of hippocampal slices**

### Files and variables

#### File: Electrophysiology\_RAW\_DATA.xlsx

**Description:** The effects of VH-N412 on seizure activity led us to question whether the conjugate could modulate hippocampal neuronal hyperactivity induced by KA. We addressed this question using acute hippocampal slices that were recorded in the presence or absence of KA and VH-N412.

##### Variables

* Mean values of the normalized spike rates (± SEM) were plotted as a function of time measured in minutes.

## Access information

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临床前及临床研究表明，轻至中度亚低温对心脏骤停、缺血性卒中、围产期缺氧/缺血、创伤性脑损伤及癫痫发作具有神经保护作用。亚低温的诱导主要依赖物理降温疗法，但该疗法易引发多种临床并发症；而部分分子已被证实可有效实现药物诱导亚低温（pharmacologically induced hypothermia, PIH）。神经降压素（neurotensin, NT）是一种由13个氨基酸组成的神经肽，可调节体温，通过与多种受体相互作用介导其外周及中枢效应。NT经脑内给药时可诱导PIH，但外周给药时因快速降解及血脑屏障（blood brain barrier, BBB）通透性差而无法观察到该效应。我们将NT与低密度脂蛋白受体（low-density lipoprotein receptor, LDLR）结合肽进行共轭，生成具有增强BBB通透性的“载体化”NT形式。

海马切片（厚度350 μm）取自Sprague Dawley大鼠（n=3，3-4周龄，购自Janvier Laboratories），并使用振动切片机（Leica VT1200S）在冰浴、氧合的改良人工脑脊液（artificial cerebrospinal fluid, ACSF）中制备；该ACSF持续通入95% O₂与5% CO₂混合气体，成分包括葡萄糖11 mM、碳酸氢钠25 mM、氯化钠126 mM、氯化钾3.5 mM、磷酸二氢钠1.2 mM、氯化镁1.3 mM及氯化钙2 mM。切片随后在ACSF中于室温（room temperature, RT）孵育至少1小时。采用多电极阵列（multielectrode arrays, MEA）对海马切片进行记录。使用蠕动泵以3 mL/min的速率向切片持续灌注氧合ACSF（MEA chamber体积约1 mL）。向灌注液中加入KA（300 nM）及VH-N412（0.1、1、10 μm），以评估VH-N412对KA诱导神经元放电增强的影响。MEA chamber内溶液的完全置换可在换液后20秒内完成。灌注液在进入MEA chamber前通过加热装置持续预热至37°C...

# 海马切片多电极电生理记录原始数据

[https://doi.org/10.5061/dryad.vdncjsz5k]

## 数据及文件结构描述

**海马切片多电极记录原始数据**

### 文件与变量

#### 文件：Electrophysiology_RAW_DATA.xlsx

**描述**：VH-N412对癫痫发作活动的影响促使我们探究该共轭物是否可调节KA诱导的海马神经元过度兴奋。我们通过记录存在或不存在KA及VH-N412条件下的急性海马切片来解答此问题。

##### 变量

* 标准化放电频率（spike rate）的均值（±标准误，standard error of the mean, SEM）随时间（分钟）变化的曲线

## 访问信息

NA