mRNA seq for determining differential expression of mRNAs in the heart in cancer cachexia

Seven week C57BL6 male mice were injected with LLC1 lung adenocarcinoma cells or vehicle. Fourteen days later cardiac tissue was harvested for RNA isolation. Control or experimental male mice received vehicle or tumor cells. Fourteen days following implantation of cells or administration of vehicle, cardiac function was assessed by echocardiography and following euthanasia ventricular tissue was dissected for isolation of RNA.

7周龄雄性C57BL6小鼠（C57BL6 mice）分别注射LLC1肺腺癌细胞（LLC1 lung adenocarcinoma cells）或载体（vehicle）。造模14天后，采集心脏组织用于RNA提取（RNA isolation）。对照组与实验组雄性小鼠分别接受载体或肿瘤细胞处理。在细胞植入或载体给药14天后，通过超声心动图（echocardiography）评估小鼠心脏功能，安乐死后解剖心室组织（ventricular tissue）以分离RNA。