The application of Compont gel in chronic obstructive jaundice rats model

Abstract Purpose: To establish a new rat model, the pathogenesis of which is closer to the clinical occurrence of chronic obstructive jaundice with liver fibrosis. Methods: 90 SD rats were randomly divided into 3 groups. Group A common bile duct ligation, group B common bile duct injection compont and group C injection saline. The serum of three groups was extracted, and the liver function was detected by ELISA. HE staining, Masson staining and immunohistochemistry were used to detect liver pathology. Results: Group B showed a fluctuant development of jaundice, obstructive degree reached a peak at 2 weeks, and decreased from 3 weeks. HA, LA and PCIII were significantly higher than control group. 3 weeks after surgery, liver tissue fibrosis occurred in group B, and a wide range of fiber spacing was formed at 5 weeks. Immunohistochemistry showed that hepatic stellate cells were more active than the control group. Conclusion: Intra-biliary injection of Compont gel is different from the classic obstructive jaundice animal model caused by classic bile duct ligation, which can provide an ideal rat model of chronic obstructive jaundice with liver fibrosis.

摘要
目的：构建一种新型大鼠模型，使其发病机制更贴近慢性梗阻性黄疸伴肝纤维化的临床发生过程。
方法：将90只SD大鼠随机分为3组。A组行胆总管结扎术，B组行胆总管内注射Compont凝胶，C组注射生理盐水。采集三组大鼠血清，采用酶联免疫吸附试验（ELISA）检测肝功能；通过苏木精-伊红染色（HE）、马松染色（Masson）及免疫组化技术检测肝脏病理变化。
结果：B组大鼠黄疸呈波动性进展，梗阻程度于术后2周达到峰值，3周后逐步降低。透明质酸（HA）、层粘连蛋白（LA）及Ⅲ型前胶原（PCIII）水平均显著高于对照组。术后3周，B组大鼠肝组织出现纤维化改变，术后5周时形成广泛的纤维间隔。免疫组化结果显示，肝星状细胞活性显著高于对照组。
结论：胆总管内注射Compont凝胶的造模方式与经典的胆总管结扎法所致梗阻性黄疸动物模型存在差异，可构建理想的慢性梗阻性黄疸伴肝纤维化大鼠模型。