Exposure of rat hepatocytes to a variety of toxicants, assayed by CodeLink microarray.. Rattus norvegicus

DrugMatrix is a comprehensive rat toxicogenomics database and analysis tool developed to facilitate the integration of toxicogenomics into hazard assessment. Using the whole genome and a diverse set of compounds allows a comprehensive view of most pharmacological and toxicological questions and is applicable to other situations such as disease and development. Overall design: Complete Drug Matrix dataset for rat hepatocytes. Approximately 600 different compounds were profiled in up to 8 different rat tissues by obtaining tissue samples from test compound-treated and vehicle control-treated rats in biological triplicates for gene expression analysis after 0.25, 1, 3, and 5 days of exposure with daily dosing. In a few studies (1.8%), 7 days of exposure was substituted for 5 days of exposure. Samples were hybridized to CodeLink RU1 10K rat arrays (Amersham Biosciences).

DrugMatrix是一款综合性大鼠毒理基因组学（toxicogenomics）数据库与分析工具，专为推动毒理基因组学融入危险度评估流程而开发。该数据集依托全基因组与多样化化合物库，可全面覆盖多数药理学与毒理学相关问题，同时可推广应用于疾病研究、发育研究等其他场景。
整体实验设计：本数据集为针对大鼠肝细胞的完整DrugMatrix数据集。研究对约600种不同化合物在最多8种大鼠组织中完成了表达谱分析：通过对经受试化合物处理与赋形剂对照处理的大鼠采集组织样本，设置生物学重复三次，在每日给药、染毒0.25天、1天、3天及5天后进行基因表达分析。在少数（1.8%）实验中，以7天染毒替代5天染毒。所有样本均与安玛西亚生物科学（Amersham Biosciences）生产的CodeLink RU1 10K大鼠基因芯片进行杂交。