The number of offspring produced by female Drosophila simulans over 4 days following artificial transinfection of Spiroplasma, compared to uninfected controls.

<b>Description of data</b>: The dataset is a record of the number of adult offspring produced by F2 Spiroplasma-transinfected and -uninfected females. For each day there is a record of the number of males and females that emerged, followed by the total number of flies emerged. The D. simulans strain used was F15, which is infected with Wolbachia. Data is in xlsl format. <br><br><b>Description of methods for data collection</b>: Microinjections were carried out as described by Nakayama et al, 2015. The number of adult offspring produced by the F2 generation of infected parental females was counted. Each F2 female was placed with two uninfected males in a vial of fly medium to mate. They were moved into fresh vials every day for five days to allow continuous oviposition. On the sixth day, adult flies were removed, and the females were screened for Spiroplasma. The eclosed offspring from days 2 to 5 were counted. <br><br><b>Column headings</b>:<br><b>Column A</b>- Group: Independent D. simulans lines with alternative nomenclature (a, b, c and d) for easy ordering in R.<br><b>Column B</b>- Line: Independent D. simulans lines (b, c1 and c2) derived from successfully transinfected females and an uninfected control (control).<br><b>Column C</b>- Infection.status: Spiroplasma infection status of D. simulans. Infected (I) and not infected (NI).<br><b>Column D</b>- Block: Spiroplasma infection status of D. simulans. Spiroplasma positive (S+) and Spiroplasma negative (S-).<br><b>Column E</b>- Genotype: lineage (g, i, j, k, c, e, b, d, h and f) of F2 mothers.<br><b>Column F</b>- Rep: replicate female F2 D. melanogaster<br><b>Column G</b>- D2M: adult males from the second day of the fecundity assay<br><b>Column H</b>- D2F: adult females from the second day of the fecundity assay<br><b>Column I</b>- D2T: total number of males and females from the second day of the fecundity assay<br><b>Column J</b>- D3M: adult males from the third day of the fecundity assay<br><b>Column K</b>- D3F: adult females from the third day of the fecundity assay<br><b>Column L</b>- D3T: total number of males and females from the third day of the fecundity assay <br><b>Column M</b>- D4M: adult males from the fourth day of the fecundity assay<br><b>Column N</b>- D4F: adult females from the fourth day of the fecundity assay <br><b>Column O</b>- D4T: total number of males and females from the fourth day of the fecundity assay<br><b>Column P</b>- D5M: adult males from the fifth day of the fecundity assay <br><b>Column Q</b>- D5F: adult females from the fifth day of the fecundity assay<b>Total</b>: total number of adult flies produced from each female Spiroplasma-infected and -uninfected D. simulans.<br><br>

<b>数据集描述</b>: 本数据集记录了F2代螺原体（Spiroplasma）感染与未感染雌性拟暗果蝇（D. simulans）所产生的成年子代数量。每日均记录当日羽化的雄性、雌性果蝇数量，以及总羽化果蝇数。本次实验所用的拟暗果蝇品系为F15，该品系已感染沃尔巴克氏体（Wolbachia）。数据集格式为XLSX。<br><br><b>数据收集方法描述</b>: 显微注射操作参照Nakayama等人2015年的研究方法开展。统计感染亲代雌性的F2代所产生的成年子代数量。将每只F2代雌性果蝇与2只未感染的雄性果蝇置于装有果蝇培养基的试管中进行交配。每日将雌蝇转移至新鲜培养基试管中，持续5天以保证持续产卵。第6天时移除成年果蝇，并对雌蝇进行螺原体感染状态筛查。统计第2至5天羽化的子代果蝇数量。<br><br><b>列标题说明</b>:<br><b>列A</b>- 分组（Group）: 采用替代命名（a、b、c、d）的独立拟暗果蝇品系，便于在R语言中进行排序。<br><b>列B</b>- 品系（Line）: 独立拟暗果蝇品系，包括来自成功感染螺原体的雌性果蝇衍生的品系（b、c1、c2）以及未感染对照组（control）。<br><b>列C</b>- 感染状态（Infection.status）: 拟暗果蝇的螺原体感染状态，分为感染组（I）与未感染组（NI）。<br><b>列D</b>- 区组（Block）: 拟暗果蝇的螺原体感染状态，分为螺原体阳性组（S+）与螺原体阴性组（S-）。<br><b>列E</b>- 基因型（Genotype）: F2代母本的谱系（g、i、j、k、c、e、b、d、h、f）。<br><b>列F</b>- 重复样本（Rep）: 重复实验的F2代雌性黑腹果蝇（D. melanogaster）。<br><b>列G</b>- D2M: 繁殖力检测第2天的成年雄性果蝇数量。<br><b>列H</b>- D2F: 繁殖力检测第2天的成年雌性果蝇数量。<br><b>列I</b>- D2T: 繁殖力检测第2天的雌雄果蝇总数量。<br><b>列J</b>- D3M: 繁殖力检测第3天的成年雄性果蝇数量。<br><b>列K</b>- D3F: 繁殖力检测第3天的成年雌性果蝇数量。<br><b>列L</b>- D3T: 繁殖力检测第3天的雌雄果蝇总数量。<br><b>列M</b>- D4M: 繁殖力检测第4天的成年雄性果蝇数量。<br><b>列N</b>- D4F: 繁殖力检测第4天的成年雌性果蝇数量。<br><b>列O</b>- D4T: 繁殖力检测第4天的雌雄果蝇总数量。<br><b>列P</b>- D5M: 繁殖力检测第5天的成年雄性果蝇数量。<br><b>列Q</b>- D5F: 繁殖力检测第5天的成年雌性果蝇数量。<br><b>总计（Total）</b>: 每只感染螺原体与未感染螺原体的拟暗果蝇雌性所产生的成年果蝇总数量。