Transcriptome analysis of Cdc73 deletion in AML cells

The Polymerase Associated Factor (PAFc) complex is an epigenetic regulating complex that has been shown to to be important for Acute Myeloid Leukemias harboring an MLL chromosomal translocations, such as MLL-AF9 leukemias. This study describes the transcriptomic profiling of AML cells following genetic deletion of the PAFc subunit Cdc73. Overall design: Cdc73floxed cells were transformed to an AML using MLL-AF9 oncogene transduction. The cells were also transduced with a 4OHT inducible CreER. The cells were then treated for 24 or 48 hours with 4OHT to induce genetic excision of Cdc73 and polyA mRNA was isolated for sequencing of the transcriptome. Biological duplicates are labelled _1 and _2.

聚合酶相关因子复合物（Polymerase Associated Factor, PAFc）是一类表观遗传调控复合物，已被证实对携带MLL染色体易位的急性髓系白血病（Acute Myeloid Leukemia, AML）具有关键调控作用，例如MLL-AF9型白血病。本研究针对经遗传敲除PAFc亚基Cdc73后的AML细胞开展了转录组谱分析。实验设计概述：通过MLL-AF9癌基因转导，将携带Cdc73 flox等位基因的细胞转化为AML细胞；同时，该细胞还被转导了可被4-羟基他莫昔芬（4-Hydroxytamoxifen, 4OHT）诱导的CreER重组酶。随后用4OHT处理细胞24或48小时，以诱导Cdc73的遗传敲除，随后分离聚腺苷酸化mRNA（polyA mRNA）用于转录组测序。生物学重复样本分别以_1和_2作为标识。