ExtFig3A_RACE_TUBA1B_R3_LG293.tiff
收藏DataCite Commons2023-01-19 更新2024-08-18 收录
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https://figshare.com/articles/dataset/ExtFig3A_RACE_TUBA1B_R3_LG293_tiff/21804570
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<em>Xrn1 knock out A549 cells were infected with WT PR8 or PR8-PA(∆X), or mock infected. 5’ RACE was then performed using primers specific for BCAP31, TUBA1B, INSIG1 or SLC7A5, positioned ~ 200-300 nucleotides downstream of the predicted cut sites.The PCR products were run on an agarose gel. </em>
将Xrn1基因敲除的A549细胞分别感染野生型（Wild Type, WT）PR8毒株或PR8-PA(ΔX)毒株，或施加假感染处理。随后以针对BCAP31、TUBA1B、INSIG1及SLC7A5的特异性引物开展5'末端快速扩增（5' RACE）实验，引物结合位点位于预测切割位点下游约200~300核苷酸位置。最后将PCR扩增产物进行琼脂糖凝胶电泳检测。
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figshare
创建时间：
2023-01-19
