Identification of MITF-regulated genes in the human melanoma cell line Ma-Mel-15

We analyzed the transcriptional response of the human melanoma cell line Ma-Mel-15 either transfected with control siRNA (siNT = non-targeting siRNA) or transfected with siRNAs (pool of 4 active and independent siRNAs) directed against the melanocytic transcription factor and lineage oncogene MITF (Microphthalmia-associated transcription factor). The experiment was performed as biological duplicates and RNA was isolated 48 hours after siRNA transfection. We aimed to determine novel markers and pathways of melanoma cell plasticity. Total RNA was obtained from siRNA-treated Ma-Mel-15 melanoma cell lines and global gene expression profiling was done using the Illumina Human HT12 v4 platform.

本研究对人黑色素瘤细胞系Ma-Mel-15的转录应答展开分析：该细胞系分别被转染对照小干扰RNA（siNT = 非靶向siRNA），或转染靶向黑素细胞转录因子及谱系致癌基因MITF（Microphthalmia-associated transcription factor，小眼症相关转录因子）的siRNA（由4条活性独立的siRNA混合制备）。实验设置生物学重复，于siRNA转染48小时后提取总RNA。本研究旨在探明黑色素瘤细胞可塑性的新型标志物及调控通路。研究从经siRNA处理的Ma-Mel-15黑色素瘤细胞系中获取总RNA，并采用Illumina Human HT12 v4平台完成全基因表达谱分析。