Transcriptomic analysis of porcine granulosa cells cocultured with COCs and exposed to the high level of NEFAs during IVM. Transcriptomic analysis of porcine granulosa cells cocultured with COCs and exposed to the high level of NEFAs during IVM

The transcriptome pattern in cocultured granulosa cells that exposed to the high level of NEFAs duing IVM was investigated. Genome-wide transcriptome analysis was performed by microarray using EDMA platform. Overall design: Two-conditions experiment, 4 replicates of granulosa cells from 4 IVMs

本研究探究了体外成熟（IVM，in vitro maturation）过程中暴露于高水平非酯化脂肪酸（NEFAs，Non-esterified Fatty Acids）的共培养颗粒细胞的转录组（transcriptome）特征。本研究采用搭载EDMA平台的基因芯片技术开展全基因组转录组分析。实验设计概述：本实验为双条件实验，样本为来自4次体外成熟实验的颗粒细胞，共设置4个生物学重复。