Induced oleoresin biosynthesis in grand fir as a defense against bark beetles.

Grand fir (Abies grandis) saplings and derived cell cultures are useful systems for studying the regulation of defensive oleoresinosis in conifers, a process involving both the constitutive accumulation of resin (pitch) in specialized secretory structures and the induced production of monoterpene olefins (turpentine) and diterpene resin acids (rosin) by nonspecialized cells at the site of injury. The pathways and enzymes involved in monoterpene and diterpene resin acid biosynthesis are described, as are the coinduction kinetics following stem injury as determined by resin analysis, enzyme activity measurements, and immunoblotting. The effects of seasonal development, light deprivation, and water stress on constitutive and wound-induced oleoresinosis are reported. Future efforts, including a PCR-based cloning strategy, to define signal transduction in the wound response and the resulting gene activation processes are delineated. IMAGES:

巨冷杉（Grand fir，Abies grandis）的幼苗及其衍生细胞培养物，是研究针叶树防御性油脂树脂生成（oleoresinosis）的理想实验体系。
该防御过程包含双重机制：其一为在特化分泌结构中组成型积累树脂（松脂，pitch）；其二为在损伤位点的非特化细胞中诱导产生单萜烯烃（monoterpene olefins，松节油，turpentine）与二萜树脂酸（diterpene resin acids，松香，rosin）。
本数据集已阐明单萜与二萜树脂酸生物合成所涉及的代谢通路及关键酶类，并通过树脂分析、酶活性测定与免疫印迹（immunoblotting）实验，明确了茎部损伤后的共诱导动力学特征。
此外，研究还报道了季节发育、遮光处理与水分胁迫对组成型及创伤诱导型油脂树脂生成的调控效应。
后续研究规划包括采用基于PCR的克隆策略，解析损伤响应中的信号转导及后续基因激活过程，相关内容已在本数据集中概述。
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