Microbial community structure is affected by cropping sequences and bio-covers under long-term no-tillage

Changes in soil bacterial community composition were assessed in response to cropping sequences and bio-covers at long-term no-tillage sites. Main effects of 4 four different cropping sequences of corn (Zea mays L.), cotton (Gossypium hirsutum L.), and soybean (Glycine max L.) were rotated in four year phases for 12-yrs at two Tennessee Research and Education Centers in a randomized complete block design with split-block treatments of four winter bio-covers: hairy vetch (Vicia villosa L.), wheat (Triticum aestivum L.), poultry litter, and a fallow control. Using Illumina high-throughput sequencing of 16S rRNA genes, bacterial community composition was determined.

本研究针对长期免耕样地，评估种植轮作序列与生物覆盖措施对土壤细菌群落组成的影响。实验在田纳西州的两个研究与教育中心开展，采用随机完全区组设计，以4种不同的玉米（Zea mays L.）、棉花（Gossypium hirsutum L.）与大豆（Glycine max L.）种植轮作序列为主处理，该轮作以4年为一个周期，累计实施12年；并设置4种冬季生物覆盖的裂区处理：毛叶苕子（Vicia villosa L.）、小麦（Triticum aestivum L.）、家禽垫料以及休耕对照组。通过对16S rRNA基因进行Illumina高通量测序，测定土壤细菌群落组成。