Effect of STAT3-/+ secretome in CD8+ T cells in vitro culture. Effect of STAT3-/+ secretome in CD8+ T cells in vitro culture

To investigate the effect of the addition of STAT3-/+ astrocyte derived-conditioned medium in CD8+ T cells previously activated in vitro We then performed gene expression profiling analysis using data obtained from RNA-seq of 3 CD8+ T cells cultures of STAT3- conditon and 3 CD8+ T cells cultures of STAT3+ conditon Overall design: Comparative gene expression profiling analysis of RNA-seq data for CD8+ T cells + STAT3-/+ conditioned medium

为探究添加STAT3缺陷/野生型星形胶质细胞来源条件培养基对体外预激活CD8+ T细胞的影响，我们利用STAT3缺陷组与STAT3正常组各3份CD8+ T细胞培养物的RNA测序（RNA-seq）数据开展基因表达谱分析。实验整体设计：针对添加STAT3缺陷/野生型条件培养基的CD8+ T细胞的RNA测序数据，进行对比基因表达谱分析。