Expression data from uterus of high vs low prolificacy Iberian x Meishan F2 sows at 30-32 days of gestation. Sus scrofa

An F2 cross between two highly divergent porcine breeds for most productive traits, including prolificacy, was generated. F2 sows were classified as of high or low prolificacy based on phenotypic records of four consecutive parities (total number of piglets born and number of piglets born alive were recorded). At the fifth gestation, sows were slaughtered at day 30-32 of gestation, and the number of corpora lutea and number of embryos were registered. Samples from different tissues were snap frozen in liquid nitrogen for further studies. Uterus samples were used for total RNA extraction and hybridized on the affymetrix porcine genechip for comparison between high and low prolificacy F2 sows. Overall design: Uterus samples from five high and 9 low prolificacy Iberian x Meishan F2 sows were hybridized to the affymetrix porcine genechip for determination of differential gene expression.

本研究构建了两个在多数生产性状（含产仔性能）上高度分化的猪品种间的F2代杂交群体。研究人员依据4个连续胎次的表型记录（记录总产仔数与活产仔数），将F2代母猪划分为高产仔性能组与低产仔性能组。在第5胎妊娠阶段，于妊娠30至32日龄屠宰母猪，记录其黄体数与胚胎数。采集不同组织样本并经液氮快速冷冻保存，以备后续实验使用。子宫组织样本用于总RNA提取，并与Affymetrix猪基因芯片（Affymetrix porcine genechip）进行杂交，以对比高产仔与低产仔性能的F2代母猪间的基因表达差异。实验整体设计如下：选取5头高产仔性能与9头低产仔性能的伊比利亚×梅山F2代母猪的子宫组织样本，与Affymetrix猪基因芯片进行杂交，以检测差异基因表达情况。