Table_2_Analysis of lncRNA in the skeletal muscle of rabbits at different developmental stages.XLSX

It is universally acknowledged that lncRNA plays an important role in the regulation of animal skeletal muscle development regulation. However, there is a lack of relevant research on lncRNA in rabbit skeletal muscle development. Thus, we explored the expression profiles of lncRNA in rabbits at three growth stages (2-week-old fetus, 6-week-old post-weaning, and 6-month-old adult) using RNA-seq. A total of 554 differentially expressed lncRNAs (235 up- and 319 down-regulated) were found between the post-weaning and fetus groups and 19 (7 up- and 12 down-regulated) between the post-weaning and adult groups and 429 (115 up- and 314 down-regulated) between the fetus and adult. The enrichment pathways in the post-weaning and fetus groups were mainly concentrated at AMPK and PI3K-Akt signaling pathways, and the co-expression results revealed that LINC-2903, LINC-2374, LINC-8591 plays a role in early maintenance of skeletal muscle development. The enriched pathways in the fetus and adult groups were mainly involved in PI3K-Akt signaling pathways with a strong association found in mTOR signaling pathways. Analysis of the co-expression results suggests that LINC-5617 may be involved in the proliferation of embryonic skeletal muscle cells, and that LINC-8613 and LINC-8705 may provide energy for postnatal skeletal muscle development. The specific roles of different lncRNAs in different developmental stages of New Zealand White rabbits obtained. This will contribute to the subsequent study on the regulatory mechanism of muscle development in New Zealand White rabbits.

众所周知，长链非编码RNA（long non-coding RNA，lncRNA）在动物骨骼肌发育调控过程中发挥着关键作用。然而，当前针对兔骨骼肌发育中lncRNA的相关研究仍较为匮乏。为此，本研究采用RNA测序（RNA-seq）技术，对新西兰白兔（New Zealand White rabbits）3个生长阶段（胎龄2周、断奶后6周以及成年6个月）的lncRNA表达谱进行了分析。研究共鉴定得到554个差异表达lncRNA：断奶后组与胎组间存在235个上调、319个下调的差异lncRNA；断奶后组与成年组间存在19个差异lncRNA（7个上调、12个下调）；胎组与成年组间存在429个差异lncRNA（115个上调、314个下调）。断奶后组与胎组的富集通路主要集中于腺苷酸活化蛋白激酶（AMPK）信号通路与磷脂酰肌醇3-激酶-蛋白激酶B（PI3K-Akt）信号通路；共表达分析结果显示，LINC-2903、LINC-2374、LINC-8591在骨骼肌发育早期维持过程中发挥作用。胎组与成年组的富集通路主要涉及PI3K-Akt信号通路，且与哺乳动物雷帕霉素靶蛋白（mTOR）信号通路存在强相关性。共表达分析进一步提示，LINC-5617可能参与胚胎期骨骼肌细胞的增殖过程，而LINC-8613与LINC-8705可能为出生后骨骼肌发育提供能量支持。本研究明确了不同lncRNA在新西兰白兔不同发育阶段的具体作用，可为后续解析新西兰白兔肌肉发育的调控机制提供重要理论支撑。