Data Sheet 1_Whole-cell response of coronavirus-infected BMDCs through proteomic and transcriptomic analyses.pdf

IntroductionUnderstanding the intricacies of the host inflammatory response to coronaviruses is essential for developing effective therapeutic strategies to mitigate the severe consequences of these infections. Various coronaviruses can trigger the host immune response, leading to highly similar inflammatory reactions. The mouse hepatitis virus (MHV), which belongs to the same group of beta-coronaviruses as SARS-CoV-2 and induces high pathogenicity in mice, typically serves as a safety model for investigating highly pathogenic coronavirus infections, replication, and virus-host interactions.
MethodsIn this study, we conducted a comprehensive analysis of the transcriptome and proteome of mouse bone marrow dendritic cells (BMDCs) infected with MHV.
ResultsWe characterized the global gene changes at both the mRNA and protein levels following viral infection, identifying ten genes involved in various anti-MHV biological processes. Furthermore, by integrating our findings with relevant published data on SARS-CoV-2 infection in cells, we observed significant similarities in the responses to MHV and SARS-CoV-2, particularly regarding immune and inflammatory responses.
DiscussionThese findings underscore how our research enhances the understanding of global gene expression alterations during coronavirus infection and facilitates the identification of novel antiviral targets.

引言
深入解析宿主对冠状病毒的炎症反应机制，对开发有效治疗策略以缓解此类感染的严重危害至关重要。多种冠状病毒均可触发宿主免疫应答，引发高度相似的炎症反应。鼠肝炎病毒（mouse hepatitis virus, MHV）与SARS-CoV-2同属β冠状病毒属，可在小鼠体内诱导高致病性，通常作为研究高致病性冠状病毒感染、复制及病毒-宿主相互作用的安全模型。

材料与方法
本研究对感染MHV的小鼠骨髓树突状细胞（mouse bone marrow dendritic cells, BMDCs）开展了转录组与蛋白质组的综合分析。

结果
本研究表征了病毒感染后mRNA与蛋白质水平的全局基因表达变化，鉴定出10个参与不同抗MHV生物学过程的基因。此外，通过将本研究结果与已发表的SARS-CoV-2细胞感染相关数据进行整合分析，我们发现MHV与SARS-CoV-2的宿主应答存在显著相似性，尤其在免疫与炎症应答领域。

讨论
本研究结果进一步强化了我们对冠状病毒感染过程中全局基因表达改变的认知，并为新型抗病毒靶点的筛选提供了重要支撑。