Supplementary Material for: Development of a linkage map and QTL scan for growth traits in North American bison

PCR protocols incorporating fluorescently labeled multiplexed primer combinations were developed to produce a linkage map for bison. Three hundred fifty eight microsatellite loci spanning all 29 autosomes were genotyped via 83 PCR multiplexes and nine individual amplifications. A total of 292 markers were integrated into an autosomal linkage map for bison. The sex averaged bison map (2,647 cM) was approximately 9% longer than the corresponding USDA MARC map, which covered 2,415 cM. Utilizing weaning, yearling and 17-month weights from two private bison herds, a QTL scan was conducted using the developed linkage map. LOD peaks suggestive of QTL were identified on chromosomes 2, 7, 15, and 24 for weaning weight, chromosomes 4, 14, and 15 for yearling weight and chromosomes 8, 14, and 25 for 17-month weight. Four of the identified chromosomes have conserved synteny with regions harboring growth QTL in cattle.

本研究开发了采用荧光标记多重引物组合的聚合酶链式反应（PCR）方案，用于构建野牛的遗传连锁图谱。研究通过83组PCR多重扩增体系与9次单重扩增反应，对覆盖全部29条常染色体的358个微卫星位点完成了基因分型。最终共有292个分子标记被整合至野牛常染色体连锁图谱中。该野牛性别平均连锁图谱长度为2647厘摩（cM），较覆盖长度为2415 cM的美国农业部肉类动物研究中心（USDA MARC）参考图谱长约9%。研究借助两个私有野牛种群的断奶体重、周岁体重及17月龄体重数据，依托本研究构建的连锁图谱开展了数量性状位点（QTL）扫描，结果显示与断奶体重相关的提示性QTL的LOD值峰值分布于2号、7号、15号及24号染色体，与周岁体重相关的峰值分布于4号、14号及15号染色体，与17月龄体重相关的峰值分布于8号、14号及25号染色体，其中4个已检测的染色体区域与牛中携带生长相关QTL的基因组区段存在保守同线性。