DNA-PKcs is a key transcriptional and splicing regulator of AR-Vs in advanced prostate cancer

To investigate the role of DNA-PKcs and RBMX in the regulation of androgen receptor variants we used the CWR22Rv1-AR-EK and CWR22Rv1 cell line in which DNA-PKcs or RBMX has been knocked down by siRNA or DNA-PKcs was inhibited with 1 µM NU7441, NU5455 or AZD7648. We then performed gene expression analysis using data obtained from RNA-seq at two time points (72 hours knockdown and 24 hours drug treatment). Overall design: Comparative gene expression profiling analysis of RNA-seq data for CWR22Rv1-AR-EK and CWR22Rv1 cells.

为探究DNA依赖蛋白激酶催化亚基（DNA-PKcs）与RNA结合基序蛋白X（RBMX）在雄激素受体变异体调控中的作用，本研究使用了CWR22Rv1-AR-EK与CWR22Rv1细胞系，通过小干扰RNA（siRNA）分别敲低其中DNA-PKcs或RBMX的表达，或采用1 μM的NU7441、NU5455及AZD7648抑制DNA-PKcs的活性。随后，我们针对两个时间节点（基因敲低72小时与药物处理24小时）获取的RNA测序（RNA-seq）数据开展基因表达分析。整体实验设计：对CWR22Rv1-AR-EK与CWR22Rv1细胞的RNA测序数据进行对比基因表达谱分析。