Cell cycle and P53 gate the direct conversion of human fibroblasts to dopaminergic neurons

Suppression of P53 in conjunction with cell cycle arrest at G1 and appropriate extracellular environment markedly increases the efficiency in the transdifferentiation of human fibroblasts to iDA neurons bt Ascl1, Nurr1, Lmx1a and miR-124. MRC-5 cells were used as control, iN cells generated from MRC-5 were analyzed.

联合抑制P53，并使细胞周期阻滞于G1期，同时辅以适宜的细胞外微环境，可显著提升Ascl1、Nurr1、Lmx1a及微小RNA124（miR-124）介导的人成纤维细胞向诱导型多巴胺能神经元（iDA neurons）转分化的效率。本研究以MRC-5细胞作为对照，对由MRC-5细胞诱导生成的诱导神经元（iN cells）进行了分析。