Analysis of transgenerational effects on DNA copy number aberrations in male mice exposed to continuous 20mGy/day gamma-rays for 400 days (Secondary screening for 20mGyI familly).. Mus musculus

Transgenerational effects of continuous low dose-rate (LDR) gamma-ray irradiation have not been well studied. C57BL/6JNrs male mice were maintained in non-irradiated room for 400 days from 8 weeks of age. This study investigated the De novo mutation by comparing the parents and children. This is secondary screening. This study was performed under contract with the Aomori Prefectural Government, Japan. Overall design: Two-condition experiment, C57 BL/6JNrs tail tissue non-irradiated male (20mGyIM), female (20mGyIF) and their progenies (20mGyI1 to 20mGyI7) vs. reference male mouse.

持续低剂量率（low dose-rate, LDR）γ射线辐照的跨代效应尚未得到充分研究。本研究选取C57BL/6JNrs品系雄性小鼠，自其8周龄起于无辐照环境中饲养400天。本研究通过比较亲本与子代，探究新生突变（de novo mutation）的发生情况，属于二次筛选实验。本研究系与日本青森县政府签订合同后实施。实验设计：双组对照实验，以未辐照C57 BL/6JNrs品系雄性小鼠的尾部组织（20mGyIM组）、雌性小鼠的尾部组织（20mGyIF组）及其子代的尾部组织（20mGyI1至20mGyI7组）为处理组，以普通雄性小鼠为对照。