The prominent transcriptional and metabolites changes of Mycobacterium bovis BCG str. Tokyo 172 induced by vitamin B1

Vitamin B1 (VB1) is a key dietary nutrient and crucial cofactor, which exhibits a series of regulatory functions on cellular processes and the activation of the immune system. To date, the precise effect of VB1 on Mycobacterium tuberculosis has not been fully described. In the present study, the direct influence of VB1 treatment on M. bovis BCG was determined using RNA-sequencing and LC/MS. The selection of this strain was used due to its common physiological features with M.tuberculosis. The investigation of the M. bovis BCG transcription demonstrated significant changes in certain metabolic and cellular process such as the decrease in fatty acid, cholesterol and glycolipid catabolism, the decrease in DNA replication and protein translation, the reduction in cell division and cell wall formation. LC/MS indicated that most of amino acids and ADP decreased in VB1 processed culture. In addition, growth assays indicated that VB1 inhibited the BCG growth rate in vitro. This study will be benefit for our deeply understanding for impact of VB1 to M.tuberculosis. Overall design: 2 groups (control and treated group), each group contain triplicates

维生素B1（Vitamin B1，VB1）是一类关键的膳食营养素与重要辅助因子，对细胞进程及免疫系统激活具有一系列调控功能。截至目前，VB1对结核分枝杆菌（Mycobacterium tuberculosis）的确切作用尚未得到完整阐释。本研究通过RNA测序（RNA-sequencing）与液相色谱-质谱联用（LC/MS）技术，探究了VB1处理对牛分枝杆菌卡介菌（Mycobacterium bovis BCG）的直接影响。选择该菌株是因其与结核分枝杆菌具有共通的生理特性。对牛分枝杆菌卡介菌转录组的分析显示，部分代谢与细胞进程发生显著变化：脂肪酸、胆固醇及糖脂的分解代谢减弱，DNA复制与蛋白质翻译水平下调，细胞分裂与细胞壁形成过程受到抑制。液相色谱-质谱联用分析结果显示，经VB1处理的培养物中，绝大多数氨基酸与二磷酸腺苷（ADP）含量均出现下降。此外，生长实验表明VB1在体外可抑制卡介菌的生长速率。本研究将有助于我们深入理解VB1对结核分枝杆菌的作用效应。实验整体设计：设置对照组与处理组共2组，每组均设置3个生物学重复。