Global transcriptome profiling hnRNP LL depleted 293T cells

We performed RNA-seq of 293T cells post depletion of hnRNP LL to look for changes brought about in the transcriptome. Overall design: For transcriptome analysis, 293T cells were treated with scramble siRNA pool or siRNA targetting hnRNP LL. Cells were treated for 72 hours with the siRNAs before proceeding for RNA extraction.

我们对hnRNP LL敲低后的293T细胞实施了RNA测序（RNA-seq），旨在探究其转录组的变化情况。整体实验设计：为完成转录组分析，我们将293T细胞分为两组，分别采用阴性对照siRNA混合池或靶向hnRNP LL的siRNA进行处理；两组细胞均经siRNA处理72小时后，再开展RNA提取工作。