Chronically radiation-exposed survivor GBM cells display poor response to Chk1 inhibition.. Chronically radiation-exposed survivor GBM cells display poor response to Chk1 inhibition.

To investigate radiation response of reccurent glioblastoma models after radiotherapy, we have utilized clinically relevant radioresistant cell model. We have generated to radiation survivor GBM population from 1 cell line and 1 primary cell line. To investigate transcriptomic alterations we have performed RNA-seq to our both naive and IR survivor samples as triplicates. Overall design: Examining transcriptomic alterations in radiation-survivor and naive GBM cells.

为探究复发性胶质母细胞瘤（glioblastoma, GBM）模型经放疗后的辐射应答特性，本研究选用了具备临床相关性的辐射抵抗细胞模型。我们分别从1株细胞系与1株原代细胞系中构建了辐射存活的胶质母细胞瘤细胞群体。为探究转录组学层面的改变，我们对未处理（naive）及电离辐射（IR）存活的两组样本均设置了三次生物学重复，并完成了RNA测序（RNA-seq）。整体实验设计：分析辐射存活与未处理的胶质母细胞瘤细胞的转录组差异。