Perinatal cardiomyocyte maturation reference isolated by large-particle fluorescence activated cell sorting

To date, there have been limited high quality libraries of cardiomyocyte maturation during the perinatal period, in part owing to the difficulty of isolating large perinatal cardiomyocytes. We previously developed a method utilizing large-particle fluorescent activated cell sorting (LP-FACS) to isolate adult cardiomyocytes for single cell RNA-seq (Kannan et al., Circ Res, 2019). We utilize this method to generate a reference of perinatal cardiomyocyte maturation. We isolated CMs from mice at the following timepoints using LP-FACS: e14, e18, p0, p4, p8, p11, p14, p18, p22, p28, p35, p56.

迄今为止，围产期心肌细胞（cardiomyocytes）成熟相关的高质量转录组文库仍较为稀缺，这在一定程度上源于难以分离获取大型围产期心肌细胞。我们此前曾开发一种利用大颗粒荧光激活细胞分选（large-particle fluorescent activated cell sorting, LP-FACS）技术的方法，用于分离成年心肌细胞以开展单细胞RNA测序（single cell RNA-seq）研究（Kannan 等，《循环研究》（Circ Res），2019 年）。本研究沿用该方法，构建围产期心肌细胞成熟的参考数据集。我们通过LP-FACS技术从小鼠体内分离得到心肌细胞（cardiomyocytes, CMs），采集的时间点包括：e14、e18、p0、p4、p8、p11、p14、p18、p22、p28、p35及p56。