Identification of mRNAs enriched in G3BP immunoprecipitation

A phosphorylation-dependent endoribonuclease G3BP localizes in cytoplasmic stress granules (SGs). G3BP promotes SG assembly, oligomerizes, binds mRNA, and regulates levels of certain mRNAs. Transcripts bound to G3BP were identified through expression profiling of a total of 12,135 genes by immunoprecipitation (IP) with anti-G3BP or control IgG from S2-013 cells, converting the immunoprecipitated transcripts to cDNA by reverse transcription, and hybridizing these products to cDNA microarrays. Five G3BP-associated transcripts were identified. In the study presented here, 1 immunoprecipitated sample with control IgG and 1 immunoprecipitated sample with anti-G3BP were used to acquire expression profiles of a total of 8,644 unique genes. The supplementary file 'GSE17056_higher_aG3BP.txt' lists the differentially expressed genes.

依赖磷酸化的核糖核酸内切酶G3BP定位于细胞质应激颗粒（stress granules，SGs）。G3BP可促进应激颗粒的组装、发生寡聚化、结合信使核糖核酸（mRNA），并调控特定mRNA的水平。本研究通过对S2-013细胞使用抗G3BP抗体或对照免疫球蛋白G（IgG）进行免疫沉淀（immunoprecipitation，IP），将免疫沉淀得到的转录本经逆转录转化为互补脱氧核糖核酸（cDNA），再将上述产物与cDNA微阵列进行杂交，对总计12135个基因开展表达谱分析，以此鉴定与G3BP结合的转录本，最终确定了5个G3BP相关转录本。在本研究所用的实验中，我们分别采用1份对照IgG免疫沉淀样本与1份抗G3BP免疫沉淀样本，获取了总计8644个独特基因的表达谱。补充文件‘GSE17056_higher_aG3BP.txt’中列出了本研究的差异表达基因。