Transcriptome profiling following knockdown of NAA50 in Arabidopsis. Transcriptome profiling following knockdown of NAA50 in Arabidopsis

We utilized RNA-seq to identify transcriptome changes following knockdown of the Arabidopsis N-terminal acetyltransferase NAA50. Transgenic Arabidopsis plants carrying a dexamethasone-inducible amiRNA were used to study the effects of NAA50 knockdown. Significant changes occurred in the transcriptomes of NAA50 knockdown plants at 12 and 24 hours after dexamethasone treatment. These changes demonstrate that loss of NAA50 results in a reduction in signaling related to plant growth and development, as well as an increase in the expression of stress- and defense-related pathways. Overall design: Transcriptome changes following knockdown of NAA50 were observed. A dexamethosone-inducible amiRNA targetting Arabidopsis NAA50 was utilized to achieve NAA50 knockdown. A transgenic line containing a dexamethasone-inducble scrambled amiRNA was used as a negative control. Four-week-old plants were treated with dexamethasone and RNA was collected 0, 12, and 24 hours later. RNA was collected from rosettes.

本研究通过RNA测序（RNA-seq），鉴定拟南芥N端乙酰转移酶NAA50敲低后的转录组变化。本研究采用携带地塞米松诱导型人工microRNA（amiRNA）的转基因拟南芥植株，探究NAA50敲低的生物学效应。在施加地塞米松处理后的12小时与24小时，NAA50敲低植株的转录组呈现显著变化。上述结果表明，NAA50功能缺失会削弱植物生长发育相关的信号通路活性，并上调胁迫与防御相关通路的基因表达水平。实验设计概述：本研究观测了NAA50敲低后的转录组变化。本研究使用靶向拟南芥NAA50的地塞米松诱导型amiRNA实现NAA50敲低，并设置携带地塞米松诱导型乱序amiRNA的转基因株系作为阴性对照。对生长4周的植株施加地塞米松处理，分别在处理后0、12、24小时采集莲座叶组织的RNA样本。