Local IL-10 delivery modulates the immune response and enhances repair of volumetric muscle loss muscle injury

To explore the impact of targeting IL-10 signaling, tibialis anterior (TA) VML injuries were created and then treated in Sprague Dawley rats using an autologous minced muscle regenerative medicine repair strategy in combination with delayed exogenous IL-10 delivery. We then performed gene expression profiling analysis using data obtained from RNA-seq from rat muscles (n=4 for IL-10 treatment, n=4 for PBS control , and n=4 for uninjured control) at day 14 post injury Overall design: Comparative gene expression profiling analysis of RNA-seq data from rat Tibialis anterior muscle tissues between unjured, Pbs abd IL-10 treatment

为探究靶向白细胞介素10（IL-10）信号通路的效应，本研究在斯普拉格-道利（Sprague Dawley）大鼠体内构建胫骨前肌（tibialis anterior, TA）容积性肌肉缺损（VML）损伤模型，随后采用自体碎肌再生医学修复策略联合延迟性外源性IL-10递送进行干预处理。随后，我们对损伤后第14天的大鼠肌肉组织开展RNA测序（RNA-seq），基于所得数据进行基因表达谱分析；其中IL-10处理组、磷酸盐缓冲液（Phosphate Buffered Saline, PBS）对照组及未损伤对照组每组各包含4只大鼠（n=4）。研究设计概述：本研究通过RNA测序数据，对未损伤、PBS处理及IL-10处理三组大鼠的胫骨前肌组织的基因表达谱进行比较分析。