Effects of dietary postbiotic on growth performance hematological parameters and liver transcriptome of calves

This study aimed to investigate the effect of postbiotic from dead cell Lactobacillus ingluviei CR37 on growth performance, antioxidant capacity, and liver transcriptional response of calves. Fourteen Holstein bull calves were assigned to 2 treatment groups: CON (n = 7) or DCLI (n = 7, fed 1 g/d of dead cell Lactobacillus ingluviei CR37 strain, containing 108 CFU/g). Calves received milk replacer (MR) at 1.75% of BW (based on air-dry weight) and the amount was adjusted weekly according to body weight, while fresh and clean water was provided ad libitum. All calves had free access to starter starting on day 33. On day 82, the MR solution was reduced to 50% of the previous week's allocation, and calves were completely weaned by day 89. Liver samples were collected after slaughter on day 90. Transcriptome analysis identified 33 DEGs, including 16 upregulated DEGs such as Endothelial lipase (LIPG), Peroxisomal Acyl-CoA oxidase 1 (ACOX1), Solute carrier family 27 member 6 (SLC27A6), and 17 downregulated DEGs such as Family with sequence similarity 107 member A (FAM107A), 4-Hydroxy-2-oxoglutarate aldolase 1 (HOGA1), Farnesyl diphosphate synthase (FDPS). Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis identified 11 significant pathways, including the PPAR signaling pathway and Pentose phosphate pathway. Taken together, postbiotic supplementation demonstrated potential benefits in improving lipid metabolism and mitigating oxidative stress in weaning calves. Fourteen Holstein bull calves were assigned to 2 treatment groups: CON (n = 7) or DCLI (n = 7, fed 1 g/d of dead cell Lactobacillus ingluviei CR37 strain, containing 108 CFU/g). Calves received milk replacer (MR) at 1.75% of BW (based on air-dry weight) and the amount was adjusted weekly according to body weight, while fresh and clean water was provided ad libitum. All calves had free access to starter starting on day 33. On day 82, the MR solution was reduced to 50% of the previous week's allocation, and calves were completely weaned by day 89. Liver samples were collected after slaughter on day 90.

本研究旨在探究灭活细胞英格鲁维伊乳杆菌（Lactobacillus ingluviei）CR37所产后生元（postbiotic）对犊牛生长性能、抗氧化能力及肝脏转录应答的影响。选取14头荷斯坦公犊牛，随机分为2个处理组：对照组（CON，n=7）与DCLI组（n=7，每日饲喂1 g灭活细胞英格鲁维伊乳杆菌CR37菌株，该菌株活菌数为10^8 CFU/g）。犊牛按照体重1.75%的比例（以风干重计）饲喂代乳粉（milk replacer, MR），饲喂量每周根据体重进行调整，同时自由供应新鲜洁净饮水。自第33日起，所有犊牛可自由采食开食料。第82日时，代乳粉饲喂量降至前一周的50%，至第89日时犊牛完全断奶。第90日屠宰后采集肝脏样本。转录组分析共鉴定出33个差异表达基因（differentially expressed genes, DEGs），其中16个为上调差异表达基因，包括内皮脂肪酶（Endothelial lipase, LIPG）、过氧化物酶体酰基辅酶A氧化酶1（Peroxisomal Acyl-CoA oxidase 1, ACOX1）、溶质载体家族27成员6（Solute carrier family 27 member 6, SLC27A6）；另有17个下调差异表达基因，包括序列相似性家族107成员A（Family with sequence similarity 107 member A, FAM107A）、4-羟基-2-氧戊二酸醛缩酶1（4-Hydroxy-2-oxoglutarate aldolase 1, HOGA1）、法尼基二磷酸合酶（Farnesyl diphosphate synthase, FDPS）。京都基因与基因组百科全书（Kyoto Encyclopedia of Genes and Genomes, KEGG）富集分析共鉴定出11条显著富集通路，包括PPAR信号通路与磷酸戊糖途径。综上，后生元补充剂可改善断奶犊牛的脂质代谢并缓解氧化应激，具有潜在应用价值。