Circulating miRNA profile and coronary calcification in asymptomatic patients with familial hypercholesterolemia.

Affymetrix microarray (GeneChip microRNA 4.0) profilling of circulating miRNAs. We used miRNA arrays to profile miRNAs isolated from plasma from familial hypercholesterolemia patients with (FH-W_CCS) or without (FH-WO_CCS) coronary calcium score by computed tomographic angiography We isolated circulating miRNAs from plasma samples of familial hypercholesterolemia patients and profiled them to determine miRNA signature.

本数据集采用Affymetrix微阵列（GeneChip microRNA 4.0）对循环微小RNA（circulating microRNAs, miRNAs）开展表达谱分析。我们利用miRNA芯片，对经计算机断层血管造影检测确认存在冠状动脉钙化评分（FH-W_CCS）或不存在冠状动脉钙化评分（FH-WO_CCS）的家族性高胆固醇血症患者血浆中分离得到的miRNA进行表达谱分析。我们从上述家族性高胆固醇血症患者的血浆样本中分离循环miRNA，并对其进行表达谱分析，以确定特征性miRNA表达谱。