The salivary proteome in relation to oral mucositis in autologous hematopoietic stem cell transplantation recipients: a labelled and label-free proteomics approach-DDALFQ

Decreased salivary flow rates and/or changes in protein composition reported after autologous hematopoietic stem cell transplantation (ASCT) reduces the protective function of saliva. This might be associated with the development of oral mucositis (OM), an inflammation of the oral mucosa as a result of chemotherapy before ASCT which affects patients? quality of life and risk factor for systemic infections. In this study, a TMT-labelled proteomics experiment, a label-free quantification (LFQ) proteomics experiment and a DIA-MS proteomics experiment were used to identify differences in the salivary proteome between patients with ulcerative OM (uOM; WHO score ? 2) and those without (nOM). For the TMT-labelled experiment, saliva samples of 5 uOM and 5 nOM were pooled at different time points: baseline, 1, 2, and 3 weeks after ASCT and 3 months after ASCT. A different salivary proteome was suggested by a distinct clustering of the OM pools at baseline, week 2 and week 3 after ASCT.

自体造血干细胞移植（autologous hematopoietic stem cell transplantation, ASCT）后，患者唾液流速下降和/或蛋白质组成发生改变，可降低唾液的保护功能。这可能与口腔黏膜炎（oral mucositis, OM）的发生相关：口腔黏膜炎是自体造血干细胞移植前化疗引发的口腔黏膜炎症，会降低患者的生活质量，并成为全身感染的危险因素。本研究采用TMT标记蛋白质组学实验、无标记定量（label-free quantification, LFQ）蛋白质组学实验以及数据非依赖采集质谱（DIA-MS）蛋白质组学实验，旨在鉴定溃疡性口腔黏膜炎（ulcerative OM, uOM；WHO评分≥2）患者与非口腔黏膜炎患者（nOM）的唾液蛋白质组差异。在TMT标记实验中，研究人员收集了5名溃疡性口腔黏膜炎患者与5名非口腔黏膜炎患者的唾液样本，并在基线期、自体造血干细胞移植后1、2、3周及移植后3个月等多个时间点对样本进行混合。在基线期、移植后第2周和第3周采集的口腔黏膜炎患者混合唾液样本呈现出显著的聚类特征，提示唾液蛋白质组存在明显差异。