Additional file 1 of Age, sex, and mating status discrimination in the sand fly Lutzomyia longipalpis using near infra-red spectroscopy (NIRS)

Additional file 1: Figure S1. Principal component analysis (PCA) of non-copulated Lutzomyia longipalpis females at different ages. (a) One (blue squares), 3 (brown triangles), 8 (blue stars), 10 (red circles), 15 (green triangles), and 17 (light blue diamonds) days old; (b) 1 (blue squares), 8 (red circles), and 15 (green triangles) days old; (c) < 8 (blue squares) or ≥ 8 (red circles) days old. Figure S2. Loadings of PCA [principal component 1 (PC – 1)] of non-copulated Lutzomyia longipalpis females at different ages: (a) 1-8-15 days old and (b) < 8 or ≥ 8 days old. Figure S3. Loadings of PCA [principal component 1 (PC – 1)] of non-copulated Lutzomyia longipalpis males at different ages: (a) 1-3-8-10-15-17 days old, (b) 1 8 15 days old, and (c) < 8 or ≥ 8 days old. Figure S4. Principal component analysis (PCA) of non-copulated Lutzomyia longipalpis males at different ages. (a) One (blue squares), 3 (brown triangles), 8 (gray stars), 10 (red circles), 15 (green triangles), and 17 (light blue diamonds) days old; (b) 1 (blue squares), 8 (red circles), and 15 (green triangles) days old; (c) < 8 (blue squares) or ≥ 8 (red circles) days old. Figure S5. Loadings of PCA [principal component 1 (PC – 1)] of copulated Lutzomyia longipalpis females at different ages: (a) 1-8-15 days old and (b) < 8 or > 8 days old, zoomed in. Figure S6. Loadings of PCA (principal component 1 (PC – 1)) of copulated Lutzomyia longipalpis males at different ages: (a) 1-8-15 days old and (b) < 8 or > 8 days old. Figure S7. Principal component analysis (PCA) of copulated Lutzomyia longipalpis at different ages. (a) One (blue squares), 8 (green triangles), and 15 (red circles) days old; (b) < 8 (blue triangles) or > 8 (red circles) days old. Figure S8. Principal component analysis (PCA) of copulated Lutzomyia longipalpis males at different ages. (a) One (blue squares), 8(green triangles), and 15 (red circles) days old; (b) < 8 (blue squares) or > 8 (red circles) days old. Figure S9. Principal component analysis (PCA) of copulated (CP, blue squares) vs. non copulated (NC, red circles) Lutzomyia longipalpis at the same age. (a) Females; (b) males. Figure S10. Principal component analysis (PCA) of females fed on sucrose solution (Suc, blue squares) or sucrose plus blood (Suc_B, red circles). (a) Results keeping females with unlaid eggs, (b) without females with unlaid eggs.

附加文件1：图S1。不同日龄未交配长须罗蛉（Lutzomyia longipalpis）雌性的主成分分析（PCA）。(a) 1日龄（蓝色方形）、3日龄（棕色三角形）、8日龄（蓝色星形）、10日龄（红色圆形）、15日龄（绿色三角形）及17日龄（浅蓝色菱形）样本；(b) 1日龄、8日龄及15日龄样本；(c) 日龄<8（蓝色方形）或≥8（红色圆形）样本。图S2。不同日龄未交配长须罗蛉雌性的主成分分析（PCA）载荷图[主成分1（PC-1）]：(a) 1、8、15日龄样本，(b) 日龄<8或≥8样本。图S3。不同日龄未交配长须罗蛉雄性的主成分分析（PCA）载荷图[主成分1（PC-1）]：(a) 1、3、8、10、15、17日龄样本，(b) 1、8、15日龄样本，(c) 日龄<8或≥8样本。图S4。不同日龄未交配长须罗蛉雄性的主成分分析（PCA）。(a) 1日龄（蓝色方形）、3日龄（棕色三角形）、8日龄（灰色星形）、10日龄（红色圆形）、15日龄（绿色三角形）及17日龄（浅蓝色菱形）样本；(b) 1日龄、8日龄及15日龄样本；(c) 日龄<8（蓝色方形）或≥8（红色圆形）样本。图S5。不同日龄已交配长须罗蛉雌性的主成分分析（PCA）载荷图[主成分1（PC-1）]：(a) 1、8、15日龄样本，(b) 日龄<8或>8样本（已局部放大）。图S6。不同日龄已交配长须罗蛉雄性的主成分分析（PCA）载荷图[主成分1（PC-1）]：(a) 1、8、15日龄样本，(b) 日龄<8或>8样本。图S7。不同日龄已交配长须罗蛉的主成分分析（PCA）。(a) 1日龄（蓝色方形）、8日龄（绿色三角形）及15日龄（红色圆形）样本；(b) 日龄<8（蓝色三角形）或>8（红色圆形）样本。图S8。不同日龄已交配长须罗蛉雄性的主成分分析（PCA）。(a) 1日龄（蓝色方形）、8日龄（绿色三角形）及15日龄（红色圆形）样本；(b) 日龄<8（蓝色方形）或>8（红色圆形）样本。图S9。相同日龄已交配组（CP，蓝色方形）与未交配组（NC，红色圆形）长须罗蛉的主成分分析（PCA）。(a) 雌性样本；(b) 雄性样本。图S10。饲喂蔗糖溶液（Suc，蓝色方形）或蔗糖加血液（Suc_B，红色圆形）的雌性长须罗蛉的主成分分析（PCA）。(a) 纳入未产卵雌虫的分析结果，(b) 排除未产卵雌虫的分析结果。