Read counts for most abundant microbial agent in patient samples - (Item G)

Vitreous was analyzed using metagenomics using two DNA isolation methods (QIAamp DNA Mini Kit, QIA; QIAamp UCP Pathogen Mini kit, UCP) and the taxonomic affiliation of reads was determined using Kraken, Bracken, and BLASTn analysis. <br>The read counts for the most abundant microbial agent in endophthalmitis patients are listed. <br><br><b>Reference</b> <b>Kirstahler P, Bjerrum SS, Friis-Møller A, </b><b>la Cour M, Aarestrup FM, Westh H., and Pamp SJ. (2018) </b>Genomics-Based Identification of Microorganisms in Human Ocular Body Fluid.<b> </b><b>Scientific Reports</b>, doi:10.1038/s41598-018-22416-4.

本研究采用宏基因组学（metagenomics）方法对玻璃体样本进行分析，采用两种DNA提取试剂盒（QIAamp DNA Mini Kit，简称QIA；QIAamp UCP Pathogen Mini Kit，简称UCP）开展实验，并通过Kraken、Bracken及BLASTn分析确定测序读段（reads）的分类学归属。
本数据集列出了眼内炎患者体内丰度最高的微生物的测序读段计数结果。

**参考文献**
**Kirstahler P、Bjerrum SS、Friis-Møller A、la Cour M、Aarestrup FM、Westh H及Pamp SJ（2018）。基于基因组学的人体眼体液微生物鉴定。《科学报告》，DOI:10.1038/s41598-018-22416-4。**