Additional file 2 of Characterization and Evaluation of Transgenic Rice Pyramided with the Pi Genes Pib, Pi25 and Pi54

Additional file 2 Table S1, Primers used in this study. A: Primers for constructing the vector; B: Primers for identification of the transgenes in transgenic plants; C: Primers for the Pi gene transcription analysis in RT-PCR; D: Primers for PCR amplification of the indigenous homologue alleles; E: Primers for qRT-PCR to validate the RNA-seq data; F: Primers for qRT-PCR of the selected DEGs; Table S2, DEGs identified in Kasalath; Table S3, DEGs identified in Zhenghan 10. Table S4, qRT- PCR of the selected DEGs for Kasalath; Table S5, qRT- PCR of the selected DEGs for Zhenghan 10.

附加文件2 表S1：本研究所用引物。A：载体构建引物；B：转基因植株外源基因鉴定引物；C：逆转录PCR（Reverse Transcription Polymerase Chain Reaction, RT-PCR）分析Pi基因转录水平所用引物；D：聚合酶链式反应（Polymerase Chain Reaction, PCR）扩增本地同源等位基因所用引物；E：实时定量PCR（Quantitative Real-time PCR, qRT-PCR）验证RNA测序（RNA Sequencing, RNA-seq）数据所用引物；F：实时定量PCR（Quantitative Real-time PCR, qRT-PCR）分析选定差异表达基因（Differentially Expressed Genes, DEGs）所用引物。表S2：Kasalath中鉴定得到的差异表达基因；表S3：郑旱10号中鉴定得到的差异表达基因。表S4：Kasalath选定差异表达基因的实时定量PCR验证；表S5：郑旱10号选定差异表达基因的实时定量PCR验证。