Effect of Knockdown of POSTN on gene expression of Keloid Fibroblasts

Here, we report that the knockdown of POSTN in fibroblasts leads to disruption in keloid inflammation. Moreover, molecules and pathway associated with extracellular matrix formation were impaired in POSTN-deficient fibroblasts. Therefore, our study highlights an indispensable role of POSTN in the pathogenesis of keloids. Overall design: To examine the role and possible molecular mechanisms of POSTN on keloid fibroblasts, we subjected si-POSTN and si-CTL-transfected fibroblasts for RNA-seq analysis.

本研究发现，成纤维细胞中骨膜蛋白（POSTN）的敲低会导致瘢痕疙瘩的炎症反应失调。此外，在POSTN缺陷的成纤维细胞中，与细胞外基质形成相关的分子及通路均受到损害。因此，本研究揭示了POSTN在瘢痕疙瘩发病机制中不可或缺的重要作用。整体实验设计：为探究POSTN对瘢痕疙瘩成纤维细胞的作用及其潜在分子机制，我们对转染了si-POSTN与si-CTL的成纤维细胞进行了RNA测序（RNA-seq）分析。