Circular RNA profiling of retinas in chronic ocular hypertension induced glaucoma rat models

High-throughput based circRNAs profiling was determined between retina samples from glaucoma rat models induced by episcleral vein ligation and paramagnetic microbeads injection seperately, with control rats. The sequencing results were followingly confirmed by quantitative reverse-transcription PCR. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses (KEGG) were adopted for annotation and predicting the functions of differentially expressed circRNAs. Results From this comparative circRNA analysis of retina samples from two glaucoma rat models (episcleral vein ligation and paramagnetic microbeads injnjection) and control rats, 3, 502 circRNAs were identified differentially expressed verified in two different models with the same variation trend. Of these, 697 were upregulated and 2,811 were downregulated compared to controls (both fold change > 2.0 and adjusted p-value < 0.001).

本研究针对经巩膜上静脉结扎术、顺磁性微珠注射诱导的青光眼大鼠模型，以及正常对照大鼠的视网膜样本，开展高通量环状RNA (circRNA) 表达谱分析。测序结果随后通过定量反转录PCR完成验证。本研究采用基因本体（Gene Ontology, GO）与京都基因与基因组百科全书通路富集分析（Kyoto Encyclopedia of Genes and Genomes, KEGG），对差异表达环状RNA进行功能注释与预测。通过对上述两种青光眼大鼠模型及对照大鼠的视网膜样本进行环状RNA比较分析，本研究共鉴定出3502个在两种模型中表达趋势一致的差异表达环状RNA。其中相较于对照组，有697个环状RNA表达上调、2811个环状RNA表达下调，筛选标准为倍数变化>2.0且校正后p值<0.001。