在当前研究期间生成和分析的数据集的补充材料。

Supplementary Material S1: Summary of transcriptome sequencing data, transcriptome assembly, and functional annotation of unigenes.Supplementary Material S2: Analysis of unigenes differential expression and a summary table of the data. Corresponding to the data from Fig. 1E–R.Supplementary Material S3: GO annotations analysis of DEGs. Corresponding to the data from Fig. 3C and D.Supplementary Material S4: eggNOG annotations analysis of DEGs. Corresponding to the data from Fig. 2E and F.Supplementary Material S5: KEGG enrichment analysis of DEGs classified by eggNOG for RNA processing and modification, cell motility, and nuclear structure in Sf21 cells.Supplementary Material S6: DEGs present in RIRI-PA1 and Sf21 cells across all time points.Supplementary Material S7: KEGG enrichment analysis of DEGs for the expression of recombinant protein in cells. Corresponding to the data from Fig. 3A–F.Supplementary Material S8: The protein expression-related DEGs annotated by KEGG enrichment in cells. Corresponding to the data from Fig. 3A–F, G and H.Supplementary Material S9: The intracellular environment regulation-related DEGs annotated by KEGG enrichment in cells. Corresponding to the data from Fig. 3A–F, I and J.Supplementary Material S10: Overall analysis of the KEGG enrichment of DEGs in two cell lines.Supplementary Material S11: Analysis of DEGs significantly enriched only in Sf21 cells.Supplementary Material S12: Annotation information of selected genes in the PCR experiment. Corresponding to the information in Fig. 4 and Fig. 5.Supplementary Material S13: Quantitative reverse transcription-polymerase chain reaction (qPCR) validation of RNA-seq data in RIRI-PA1 and Sf21 cells. Corresponding to the information in Fig. 4 and Fig. 5.Supplementary Material S14: The primer sequences.

补充材料S1：转录组测序数据、转录组组装及单基因功能注释汇总
补充材料S2：差异表达单基因分析及数据汇总表，对应图1E–R中的数据
补充材料S3：差异表达基因（Differentially Expressed Genes, DEGs）的基因本体（Gene Ontology, GO）注释分析，对应图3C和D中的数据
补充材料S4：差异表达基因的eggNOG注释分析，对应图2E和F中的数据
补充材料S5：针对Sf21细胞中经eggNOG分类为RNA加工与修饰、细胞运动及核结构类别的差异表达基因的京都基因与基因组百科全书（Kyoto Encyclopedia of Genes and Genomes, KEGG）富集分析
补充材料S6：全时间点下RIRI-PA1细胞与Sf21细胞中共有的差异表达基因
补充材料S7：细胞内重组蛋白表达相关差异表达基因的KEGG富集分析，对应图3A–F中的数据
补充材料S8：经KEGG富集注释的细胞内蛋白表达相关差异表达基因，对应图3A–F、G和H中的数据
补充材料S9：经KEGG富集注释的细胞内环境调控相关差异表达基因，对应图3A–F、I和J中的数据
补充材料S10：两种细胞系中差异表达基因的KEGG富集整体分析
补充材料S11：仅在Sf21细胞中显著富集的差异表达基因分析
补充材料S12：PCR实验中筛选基因的注释信息，对应图4与图5中的内容
补充材料S13：实时定量反转录聚合酶链反应（quantitative reverse transcription-polymerase chain reaction, qPCR）验证RIRI-PA1与Sf21细胞的RNA-seq数据，对应图4与图5中的内容
补充材料S14：引物序列