Gene expression changes by exogenous expression of epithelial-to-mesenchymal (EMT)-inducing transcription factors (Snail and Zeb1) and mesenchymal-to-epithelial transition (MET)-inducing transcription factor (Ovol2) in MCF10A cells. Homo sapiens

Multiple EMT-promoting/inhibiting transcription factors have been identified including Snail, Zeb1 and Ovol2. To understand differential roles of these factors, we performed gene expression profiling by RNA-seq upon Snail-/Zeb1-induced EMT or Ovol2-induced MET in human mammary epithelial MCF10A cells. Overall design: Examination of 4 samples.

目前已鉴定出多种调控上皮间质转化（Epithelial-Mesenchymal Transition, EMT）或间质上皮转化（Mesenchymal-Epithelial Transition, MET）的转录因子，包括Snail、Zeb1及Ovol2。为阐明这些因子的差异化功能，我们以人乳腺上皮MCF10A细胞为研究对象，在Snail或Zeb1诱导的EMT以及Ovol2诱导的MET模型中，通过RNA测序（RNA Sequencing, RNA-seq）开展基因表达谱分析。实验整体设计：共分析4份样本。