Role of Cterminal FGF23 peptide in inflammation

RNAseq of liver of mice with a conditional overexpression of Cterminal FGF23 peptide in osteoblasts and osteocytes and WT littermates 6 hours post-injection of 250ng/g of recombinant murine Il-1B or saline. These R26-stop-CterFgf23 mutant mice have a loxP-flanked STOP sequence followed by the Cterminal peptide of FGF23 inserted into the Gt(ROSA)26Sor locus. When bred to mice expressing Dmp1-Cre recombinase, the STOP sequence is deleted and CterFgf23 expression is observed in mature osteoblasts and osteocytes in the bone of the double mutant offspring (CterTg). At 6 weeks, WT and CterTg mice were injected with a single dose of 250ng/g recombinant murin IL-1B or saline as Ctr and sacrificed 6 hours post-injection, and Liver was collected. Total RNA was isolated and submitted for RNAsequencing.

本数据集为成骨细胞与骨细胞中条件性过表达C末端成纤维细胞生长因子23（Fibroblast Growth Factor 23, FGF23）肽段的小鼠及其野生型同窝仔鼠，在注射250ng/g重组小鼠白细胞介素1β（Interleukin-1β, IL-1B）或生理盐水6小时后的肝脏RNA测序样本。该R26-stop-CterFgf23突变小鼠的Gt(ROSA)26Sor基因座中插入了两侧带有loxP位点的终止序列，其后连接FGF23 C末端肽段的编码区；当与表达Dmp1-Cre重组酶的小鼠交配后，子代双突变体（命名为CterTg）的成熟成骨细胞与骨细胞中，终止序列被重组酶切除，从而启动CterFgf23的特异性表达。在小鼠6周龄时，对野生型与CterTg小鼠单次注射250ng/g重组小鼠IL-1B或生理盐水（作为对照组），并于注射后6小时处死小鼠，采集肝脏组织，随后提取总RNA并进行RNA测序（RNA-sequencing, RNAseq）。