Functional genomics of HDAC class-I specific inhbitor 4SC-202 in pancreatic cell lines [RNA-seq]. Homo sapiens

We examined transcriptome-wide effects of 4SC-202 in L3.6, BXPC3 and PANC1 cells as well as its effect on TGFβ signaling Overall design: We performed mRNA sequencing from L3.6, BXPC3 and PANC1 cells following following DMSO, 4SC-202 and/or TGFβ treatment. The mRNA-Seq includes following conditions: 4SC-202 vs DMSO (for L3.6, BXPC3 and PANC1 cells), TGFβ vs DMSO and 4SC-202+TGFβ vs TGFβ (for PANC1 cells). The libraries were performed in triplicates.

我们探究了4SC-202在L3.6、BXPC3及PANC1细胞中的全转录组效应，及其对转化生长因子β（TGFβ）信号通路的影响。实验整体设计：我们对经二甲基亚砜（DMSO）、4SC-202以及/或转化生长因子β（TGFβ）处理后的L3.6、BXPC3与PANC1细胞进行了mRNA测序（mRNA-Seq）。本次mRNA测序涵盖如下实验组设置：针对L3.6、BXPC3及PANC1细胞的4SC-202处理组与DMSO对照组的比较；针对全部三类细胞的TGFβ处理组与DMSO对照组的比较；以及仅针对PANC1细胞的4SC-202联合TGFβ处理组与单纯TGFβ处理组的比较。所有测序文库均设置三次生物学重复。