Data from: Genetic mapping a meiotic driver that causes sex ratio distortion in the mosquito Aedes aegypti

An endogenous meiotic driver in the dengue and yellow fever vector mosquito Aedes aegypti can cause highly male-biased sex ratio distortion in crosses from suitable genetic backgrounds. We previously selected a strain that carries a strong meiotic drive gene (D) linked with the male-determining allele (M) on chromosome 1 in A. aegypti. Here we performed segregation analysis of the MD locus among backcross (BC1) progeny from a driver male and drive-sensitive females. Assessment of sex ratios among BC2 progeny showed ~5.2% recombination between the MD locus and the sex determination locus. Multipoint linkage mapping across this region revealed consistent marker orders and recombination frequencies with the existing reference linkage map and placed the MD locus within a 6.5 cM interval defined by the LF159 locus and microsatellite marker 446GAA, which should facilitate future positional cloning efforts.

作为登革热与黄热病传播媒介的埃及伊蚊（Aedes aegypti）体内存在一种内源性减数分裂驱动因子，在适配的遗传背景下进行杂交时，该因子可引发极强的雄性偏倚性别比例畸变。我们此前已筛选获得一株埃及伊蚊品系，其1号染色体上携带有与性别决定等位基因（M）连锁的强效减数分裂驱动基因（D），该连锁位点即被称为MD位点。本研究针对驱动型雄蚊与驱动敏感型雌蚊的回交（BC1）后代开展了MD位点的分离分析。对BC2后代性别比例的评估结果显示，MD位点与性别决定位点之间的重组率约为5.2%。针对该区域开展的多点连锁作图结果显示，其标记排列顺序与重组频率与现有参考连锁图谱一致，并将MD位点定位在由LF159标记与微卫星标记446GAA界定的6.5厘摩区间内，这将为后续的位置克隆研究提供有力支撑。