MS1-Based Label-Free Proteomics Using a Quadrupole Orbitrap Mass Spectrometer.

Data from ProteomeXchange, PXD ID: PXD001385. File: QEP1_SpikeIn_230914_3_3ng_270914.mzml. Published as part of J Proteome Res. 2015 Mar 24 . From the Abstract: {{i}} Presented is a data set for benchmarking MS1-based label-free quantitative proteomics using a quadrupole orbitrap mass spectrometer. Escherichia coli digest was spiked into a HeLa digest in four different concentrations, simulating protein expression differences in a background of an unchanged complex proteome. The data set provides a unique opportunity to evaluate the proteomic platform (instrumentation and software) in its ability to perform MS1-intensity-based label-free quantification. {{/i}}

数据来源于ProteomeXchange（蛋白质组交换数据库），PXD编号为PXD001385。文件名为QEP1_SpikeIn_230914_3_3ng_270914.mzml。该数据发表于《Journal of Proteome Research》（《蛋白质组研究杂志》）2015年3月24日刊。摘要内容如下：本数据集用于基于MS1的无标记定量蛋白质组学基准测试，所用仪器为四极杆-轨道阱质谱仪。研究人员将大肠杆菌（Escherichia coli）酶解肽段以四种不同浓度掺入海拉细胞（HeLa）酶解肽段中，模拟恒定复杂蛋白质组背景下的蛋白质表达差异。本数据集为评估蛋白质组学平台（涵盖仪器与软件）基于MS1信号强度的无标记定量能力提供了独特的测试契机。