Transcriptional changes in response to TGFB in human pancreatic stellate cells with knockdown of PIN1

Pancreatic stellate cells can be activated to a myofibroblast phenotype in response to TGFB treatment. The goal of this study was to determine how loss of PIN1 affects stellate cell state at baseline and in response to TGFB treatment. Primary human pancreatic stellate cells (PSCs) were cultured in Stellate Cell Media. For knockdown studies, PSCs were infected with a pLKO-shPIN1 lentivirus or an shSCR control lentivirus. Stable pools were generated with puromycin selection. These cells were plated, and then treated for 24 hours with 5 ng/mL TGFB1. Cells were collected, RNA isolated and used for RNA sequencing.

胰腺星状细胞（Pancreatic stellate cells）可经转化生长因子β（TGFB）处理后被激活为肌成纤维细胞表型。本研究旨在明确PIN1缺失在基线状态及TGFB处理条件下对星状细胞状态的影响。原代人胰腺星状细胞（PSCs）采用星状细胞培养基（Stellate Cell Media）进行体外培养。敲低实验中，PSCs分别被pLKO-shPIN1慢病毒与shSCR对照慢病毒感染。通过嘌呤霉素筛选构建稳定细胞池。将上述细胞铺板后，以5 ng/mL 转化生长因子β1（TGFB1）处理24小时。收集细胞并提取RNA，用于RNA测序（RNA sequencing）。