Stranded RNA-seq profiling of cerebral cortices from Taf8-deleted and Taf8-intact mouse embryos

Taf8 was deleted in the mouse central nervous system using our Taf8 conditional allele. To determine transcriptional changes due to Ta8 deletion in the developing CNS, stranded RNA sequencing was performed on cortices from Taf8 deleted embryos (E14.5 Taf8lox/–NesCreT/+p53–/–) and control embryos (E14.5 Taf8+/+NesCreT/+p53–/–). Four Taf8-deleted and four control samples were prepared, each from a separate mouse embryo. Stranded RNA sequencing was undertaken on an Illumina NextSeq 500 to produce 81bp paired-end reads.

我们利用自主构建的Taf8条件性敲除等位基因（conditional allele），在小鼠中枢神经系统中实现了Taf8基因的敲除。为探究发育中的中枢神经系统内Taf8缺失引发的转录组变化，我们对Taf8敲除胚胎（胚胎发育第14.5天，基因型为Taf8lox/–NesCreT/+p53–/–）与对照胚胎（胚胎发育第14.5天，基因型为Taf8+/+NesCreT/+p53–/–）的大脑皮层组织开展了链特异性RNA测序（stranded RNA sequencing）。本次实验共制备4份Taf8敲除样本与4份对照样本，每份样本均来自独立的小鼠胚胎。测序工作在Illumina NextSeq 500测序平台上完成，最终产出81碱基对的双端测序读段（paired-end reads）。