De novo genome assembly of Candida utilis NBRC0988 and its induced genome evolution by transient DNA breaks. Cyberlindnera jadinii NBRC 0988

Genomic DNA of Candida utilis NBRC0988 was sequenced using a combination of long- and short-read sequencing technologies (PacBio Sequel and Illumina MiSeq). The reads were assembled using FALCON and MaSuRCA, and manually connected using BLAST and YASS. The control strain and mutant strains were resequenced by Illumina NovaSeq.

本研究采用长读长与短读长测序技术（PacBio Sequel与Illumina MiSeq）相结合的策略，对产朊假丝酵母（Candida utilis）NBRC0988的基因组DNA进行测序。所得测序读段通过FALCON与MaSuRCA软件完成组装，并借助BLAST与YASS工具进行手动连接。对照菌株与突变菌株均采用Illumina NovaSeq测序平台开展重测序。