Average sequencing depth of the genome sequences of the phages included in the community at different passages and lines.
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A correction was applied for pairs of closely related phages at depth ratios below 20/80 (Phages B and C). Mutations detected at passage 0 in the read mapping of each phage at a consistent specific ratio corresponded to discrepancies between the phages. We calculate the relative proportion of mapped reads corresponding to each phage by calculating the average of the frequency at which each mutation appears. We multiply this value (from 0 to 1) by the total number of reads mapped. We cannot apply this correction for pairs of close relative phages where the ratio is close to 50−50 (Phages I and J, and G and H. These phages are represented together). (XLSX)
针对深度比率低于20/80的密切相关噬菌体对(噬菌体B与C)实施了校正处理。在传代0阶段,以恒定特定比率在各噬菌体的读段映射(read mapping)分析中检测到的突变,对应于各噬菌体间的序列差异。我们通过计算每个突变出现频率的平均值,得到对应于各噬菌体的映射读段相对占比,并将该取值介于0至1之间的数值乘以映射读段的总数量。对于比率接近50−50的密切相关噬菌体对(噬菌体I与J、G与H,此类噬菌体被合并表示),无法应用该校正方法。(XLSX)
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2025-11-26



