Serum albumin coated stellate mesoporous silica nanocomposites inhibit metastatic outgrowth in zebrafish embryos

This data set is related to the present article whose abstract is given as follows : Mesoporous silica-based nanoparticles (NPs) are promising tools for developing targeted therapeutic interventions in cancer. Endowed with a large pore silica shell suitable for drug encapsulation and with a responsive magnetic core, iron oxide stellate mesoporous silica (IO STMS) NPs stand out. Yet, their impact and potential toxicity on relevant in vivo models has not been carefully tested yet. Herein, we assessed the impact of these IO STMS nanocomposites in a syngeneic metastasis assay in zebrafish. NPs were surface-modified with human serum albumin (HSA) and loaded or not with the chemotherapeutic doxorubicin (DOX). In vitro, DOX-loaded NPs were expectedly more toxic to zebrafish melanoma (Zmel) cells than no-DOX NPs. In zebrafish embryos, the NPs were rapidly distributed through blood circulation and were found to colocalize over time with the vascular endothelium and local macrophages. Suprisingly, the NPs efficiently reduced the outgrowth of Zmel tumoral masses in an experimental metastasis assay in zebrafish embryos regardless of their loading with DOX. The anti-metastatic effect of these NPs was further improved by increasing the amount of HSA coating, also resulting in higher embryo survival. Altogether, IO STMS NPs showed promising cytotoxic effects on a relevant zebrafish metastasis model, inhibiting metastatic outgrowth in vivo independently of the drug loading. This opens the door to further testing for better exploiting their targeting and drug delivery potentialities

本数据集与下述摘要对应的研究文章相关：介孔二氧化硅基纳米颗粒（mesoporous silica-based nanoparticles，NPs）是开发癌症靶向治疗手段的极具潜力的工具。氧化铁星型介孔二氧化硅（iron oxide stellate mesoporous silica，IO STMS）纳米颗粒凭借其适配药物包载的大孔径二氧化硅壳层与响应性磁核，脱颖而出。然而，目前尚未针对相关体内模型系统评估其作用与潜在毒性。本研究中，我们针对斑马鱼体内的同基因转移模型实验，评估了该IO STMS纳米复合物的作用效果。研究人员将纳米颗粒表面修饰于人血清白蛋白（human serum albumin，HSA），并分别负载与未负载化疗药物阿霉素（doxorubicin，DOX）。体外实验中，负载DOX的纳米颗粒对斑马鱼黑色素瘤（zebrafish melanoma，Zmel）细胞的毒性显著高于未负载DOX的纳米颗粒，符合预期。在斑马鱼胚胎中，纳米颗粒可通过血液循环快速分布，并随时间推移与血管内皮及局部巨噬细胞共定位。令人意外的是，无论是否负载DOX，该纳米颗粒均可在斑马鱼胚胎的实验性转移模型中有效抑制Zmel肿瘤团块的生长。增加HSA涂层的用量可进一步增强该纳米颗粒的抗转移效果，同时提升胚胎存活率。综上，IO STMS纳米颗粒在相关斑马鱼转移模型中展现出良好的细胞毒性效应，可在不依赖药物负载的情况下在体内抑制转移灶生长。这为后续进一步开发其靶向与药物递送潜力奠定了研究基础。